EGF Advances Synthetic DNA Assembly Using Fully Automated Workflow With Integrated Colony Picking From Molecular Devices

Ivan Yuan, PhD Edinburgh Genome Foundry Technologist; Hille Tekotte, PhD Edinburgh Genome Foundry Manager; Valentin Zulkower, PhD Edinburgh Genome Foundry Software Manager

The assembly of synthetic DNA has become a standard process in many scientific laboratories. The assembly protocols involve many repetitive steps and often scientists require many different versions of DNA. Therefore, this process is well suited for automation, which enables the high-throughput needed, and results in a lower error rate and cost saving for reagents and consumables.

Colony Picking is a key part of the synthetic biology workflow, where bacteria are used to propagate cloned DNA, or to screen DNA constructs against selective conditions. With high throughput, needs, the task becomes too labor-intensive and error-prone to be performed by hand.

The Edinburgh Genome Foundry (EGF) is a newly established facility of the University of Edinburgh and assembles built-to-order DNA constructs using a fully integrated robotic platform. In this webinar, EGF team members will present their robotic platform and how it performs automated DNA assembly using the Golden Gate DNA assembly method.

Attendees will gain a better understanding of how the Molecular Devices QPix system integrates into an automated production line and is used for routine high-throughput colony picking and plating. The team will delve into the customization of the instrument itself, as well as the collaborative efforts with Molecular Devices to integrate the QPix into their extensive lab automation setup.

The team at Molecular Devices has selected three industry experts from EGF to speak during this webinar; Dr. Ivan Yuan, Technologist at EGF, Dr. Hille Tekotte, Manager at EGF, and Dr. Valentin…

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